Trần Thanh Hà (hỏi) :
Sư Phụ ơi, hiện nay hình như đã có thuốc điều trị Covid 19, đó là thuốc làm tê liệt enzym RNA polymerase. Khi tiêm vào bệnh nhân, bệnh nhân phục hồi rất nhanh.
Vậy làm tê liệt enzym Polymerase RNA thì có để lại đi chứng hay hậu quả gì về sau ko ạ?
Em cảm ơn Sư Phụ nhiều ạ
Wambuakim (trả lời) :
Nhận được câu hỏi, vốn dĩ Thầy muốn trả lời ngay nhưng phải chờ thông tin chính xác từ Mỹ và châu Âu phản hồi nên đành phải bắt các bạn chờ lâu “một chút”.
1/. Bản chất sinh học của enzym Polymerase RNA :
a/. RNA polymerase (thường được gọi trong tiếng Việt là ARN pôlymêraza) là một loại enzym chuyên xúc tác quá trình tổng hợp các loại phân tử ARN từ gen. ARN Polymerase còn được gọi tên theo chức năng của nó là enzym phiên mã.
b/. ARN Polymerase có khả năng nhận biết gen khuôn mẫu tương ứng, gắn vào vùng điều hoà của gen này và tiến hành phiên mã.
Sau khi đã gắn vào vùng điều hoà của gen, ARN Polymerase có khả năng chuyển đổi trình tự poliđesoxi-ribonucleotitc (AND / poly DRN) thành trình tự chuỗi ribonucleotic (poly RN) mang mã phiên, từ đó dịch mã mới tiến hành được.
c/. Phiên mã :
Phiên mã là quá trình tổng hợp ARN. Thuật ngữ này dịch từ nguyên gốc tiếng Anh transcription, gọi theo thuật ngữ cũ là sao mã, nay đã thống nhất dịch là phiên mã
Sự tổng hợp (tạo thành) hoặc phân giải (loại bỏ) enzym ARN Polymerase có liên quan đến biểu hiện gen.
2/. Biểu hiện gen :
Biểu hiện gen là quá trình chuyển đổi thông tin di truyền chứa trong gen thành sản phẩm trong tế bào sống, từ đó tính trạng tương ứng được tạo thành ở kiểu hình có thể quan sát được. Khái niệm này được dịch từ thuật ngữ tiếng Anh: gene expression.
Ví dụ : bệnh Alzheimer (mất trí nhớ) ở người do gen trội gây ra, nhưng người mang gen trội gây bệnh này thì còn hồi trẻ thì gen này chưa biểu hiện, mãi đến 60 tuổi mới thấy bệnh phát ra.
Biểu hiện gen là quá trình rất phức tạp, trải qua nhiều giai đoạn và phụ thuộc vào rất nhiều yếu tố, trong đó có tác động của các gen khác, của môi trường trong (nội môi) và môi trường ngoài (ngoại cảnh). Tuy nhiên, ở mức độ đơn giản quá trình biểu hiện gen trải qua 3 giai đoạn chính ở cả sinh vật nhân sơ (vi khuẩn) và sinh vật nhân chuẩn (đa bào).
a/. Phiên mã: Gen tổng hợp ra ARN.
b/. Dịch mã: Mã phiên ở ARN được chuyển đổi thành chuỗi pôlypeptit.
c/. Tạo thành và hoạt động của các prôtêin chức năng.
3/. Quá trình Biểu Hiện Gen :
Biểu hiện Gen chỉ thật sự tiến hành khi có tối thiểu các nhân tố sau cùng tham gia:
a/. Xử lý ARN
Là giai đoạn chỉ diễn ra ở sinh vật nhân chuẩn, bao gồm các sự kiện:
– Gắn mũ (capping),
– Ghép mảnh (splicing) và
– Thêm đuôi polyA.
Trong đó quá trình thêm đuôi polyA liên quan mật thiết với việc kết thúc phiên mã. Tác dụng của việc gắn đuôi polyA cho mARN để giúp mARN không bị thủy phân bởi enzim khi di chuyển từ nhân ra tế bào chất để thực hiện dịch mã tổng hợp protein.
b/. Dịch mã
Dịch mã là sự tổng hợp của 1 polypeptide sử dụng thông tin trong mARN.
4/. Các kiểu biểu hiện gen :
Phần lớn thuật ngữ được dùng để miêu tả các mô hình của sự biểu hiện gen, bao gồm:
a/. Gen cấu trúc là một gen được phiên mã liên tục khi so sánh với gen tùy ý chỉ được dịch mã khi cần thiết.
b/. Gen giữ nhà (housekeeping gene) là một gen cấu trúc điển hình, được phiên mã ở một mức độ tương đối liên tục. Những sản phẩm từ sự phiên mã các gen này cần cho sự duy trì sự sống tế bào. Có thể nói là sự biểu hiện của chúng không bị ảnh hưởng bởi những điều kiện thí nghiệm. Ví dụ như actin, GAPDH và ubiquitin.
c/. Gen tuỳ ý là gen chỉ được phiên mã khi cần so sánh với gen cấu thành.
d/. Gen cảm ứng là gen mà sự biểu hiện của nó nhằm đáp ứng với những thay đổi của môi trường hay phụ thuộc vào từng trạng thái trong chu kỳ tế bào.
5/. Điều tiết biểu hiện gen :
Điều tiết biểu hiện gen là sự điều khiển tế bào về mặt số lượng và thời gian xuất hiện của các hàm chức năng của một gen. Bất kì giai đoạn nào trong biểu hiện gen cũng có thể được điều hòa, từ giai đoạn sao mã ADN-ARN đến giai đoạn biến đổi hậu phiên dịch của một protein. Điều hòa gen điều khiển tế bào về mặt cấu trúc và chức năng, và là cơ sở cho sự phân dị tế bào, đồng hình di truyền và sự linh hoạt và thích nghi của bất cứ sinh vật nào.
Do chưa có khả năng giải mật mã Gen người, cho nên sự chủ động biểu hiện Gen và điều tiết biểu hiện Gen vẫn còn là một giấc mơ của nhân loại.
6/. Nguy hiểm trong trò chơi với mật mã Gen :
Qua những dữ liệu và phân tích trên, các bạn có thể hiểu rằng trò chơi với biểu hiện Gen đã nguy hiểm đến thế nào, và trò chơi với mật mã Gen càng chứa đựng nguy hiểm triệu triệu lần hơn. Xin hãy dừng lại tất cả những trò chơi có nguy cơ hủy diệt toàn bộ sự sống hành tình, không chỉ là nhân loại yếu hèn.
7/. Đối với Virus Corona “mới”, khoa học kỹ thuật máy tính hiện đại đã tìm ra (và xác định) 77 chất liệu hóa sinh và dược pháp (với khoảng 265 combo dược chất) có thể tiêu diệt nó, không chỉ là thuốc remdesivir (mà không cần dùng đến trò chơi nguy hiểm của Enzim Polymerase RNA. Xin xem : SUMMIT, SIÊU MÁY TÍNH MẠNH NHẤT THẾ GIỚI XÁC ĐỊNH ĐƯỢC CÁC HOẠT CHẤT CÓ THỂ NGĂN CHẶN VIRUS CORONA (Covid-19) / Lớp Học Của Bác Kim / 29/3/2020).
Remdesivir có thể thỏa mãn tạm thời việc ngăn chặn Corona nhưng âm thầm dung chứa bên trong nó nguy cơ hủy diệt sự sống trên địa cầu sau 50 – 75 năm nữa..
8/. Nước Đức đã âm thầm sử dụng 26 dược pháp từ Viện Y Dược Học Lượng Tử Đức mà không công bố ra thế giới để bảo vệ công dân của nước mình từ trước những ngày bùng lên đám cháy China’s Wuhan Corona. Nhân dân Đức ra đường tự do giao thiệp mà chưa bao giờ cần đến cảnh báo khẩu trang toàn dân vừa ồn ào, vừa kệch cỡm vừa … kinh dị.
——————
Tóm lại, câu hỏi này cũng đơn giản và dễ hiểu như … chính bản thân Remdesivir. Mong các bạn sáng suốt nhận định để không phải lo âu hay bối rối không đáng.
———–Wambuakim————
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