Đăng trong bởi Trần Kim

NÔNG NGHIỆP KHÔNG PHÂN BÓN PHẦN I

PHẦN I : RHIZOBIUM AGRICULTURAL

HỎI : (Định Phương Anh)

Một lần cháu được nghe chú Kim nói về nghiên cứu giải pháp cho vi khuẩn nốt sần, không chỉ là con vi sinh mà còn là chủ nhà máy sản xuất phân đạm sinh học…..
…. Chỉ mong chú công bố nghiên cứu của mình để cháu và mọi người học hỏi.

ĐÁP : (Wambuakim)

I/. CÂY HỌ ĐẬU – NI TƠ

Phân đạm tự nhiên : Từ ngàn xưa, con người đã chú ý đến hiệu quả cải tạo đất của nhóm cây họ Đậu và Parasponia : trồng cây họ đậu gần như không cần bón phân đạm hữu cơ mà còn làm cho đất trồng giàu đạm.
Thời đại này thì chúng ta biết rõ rằng Ni tơ (khí đạm) chiếm gần 80% trong không khí, nhưng hầu hết cây trồng và sinh vật đều không thể lấy trực tiếp đạm từ không khí, do Ni tơ trong không khí liên kết bằng mối nối phân tử N2 và không có biện pháp tự nhiên nào đủ sức phá vỡ liên kết đó.

Vì nitơ trong khí quyển không ở dạng NO3- và NH4+ (dạng nitơ mà thực vật hấp thụ được) mà lại là dạng N2 (có liên kết ba rất bền vững nối giữa 2 nguyên tử nitơ). Để sử dụng được N2, sinh vật phải khử nó thành NH¬3 nhờ quá trình gọi là cố định nitơ.

Phân đạm hóa học : Năm 1920, công nghệ Haber-Bosch đã giúp con người tách mối liên kết 3 này của N2, miễn là có nguồn năng lượng có thể nâng nhiệt độ lên 400 hoặc 500oC và áp suất là 200 atmosphere. Tuy nhiên sản xuất phân bón thông qua phương pháp Haber-Bosch cho mùa màng tiêu tốn nguồn năng lượng khủng khiếp, chưa kể hiệu suất sinh học của phân bón hóa học không cao, và các tàn dư hóa học của chúng để lại trên đât trồng nhanh chóng làm cho đất bạc màu.

II/. RHIZOBIUM

Vậy mà những con vi khuẩn Rhizobuim bé tí ti ở rễ cây họ đậu lại làm được điều thần kỳ đó ở nhiệt độ thường. Chúng lấy Nitơ từ không khí và chuyển hóa qua nhiều phản ứng sinh hóa khác nhau với sự tham gia của nhiều loại emzyme để cuối cùng biến đổi nitơ trong khí quyển thành ammoniac và sau đó cung cấp các hợp chất nitơ hữu cơ như glutamin hoặc ureide cho cây. Vi khuẩn cố định đạm được phân lập năm 1988 và được nhà khoa học người Đức Fank B. đặt tên là Rhizobium năm 1989.

Một số loài vi khuẩn cố định đạm khác, như cyanbacteria, trôi nổi trong đại dương và xử lý nhiều ni-tơ đến mức chúng được công nhận là nguồn lực chính của thành phần hóa học đại dương, Nostoc cyanbacteria và vài loài địa y Lobaria. Một trong những loài nổi tiếng nhất là Gluconacetobacter diazotrophicus, nhận diện trong cây mía ở Brazil vào năm 1988, hay như vi khuẩn Frankia tạo nên những nốt nhỏ trong các cây không thuộc họ đậu, ví dụ như cây tổng quán sủi và cây thanh mai.

Quá trình cố định N2 được thực hiện nhờ các nhóm sinh vật sau:
– Vi khuẩn sống tự do:
+ Vi khuẩn hiếu khí thuộc chi: Azotobacter và Beijerinckia
+ Vi khuẩn kị khí thuộc chi: Clostridium
+ Vi khuẩn lam sống tự do thuộc chi anabaena.Tuy nhiên, sản phẩm cố định không nhiều nên ít có ứng dụng trong thực tế.
– Quá trình cố định vi khuẩn và vi khuẩn lam sống cộng sinh.
+ Vi khuẩn lam sống cộng sinh trong bèo hoa dâu: Vi dụ, vi khuẩn lam thuộc chi: Anabaena azollae.
+ Vi khuẩn Rhizobium sống cộng sinh trong rễ cây họ Đậu (như đậu cô ve, đậu tương, cỏ ba lá, linh lăng…).

Quá trình cố định nitơ nhờ vi khuẩn và vi khuẩn lam sống tự do mỗi năm có thể cố định được 10-15kg/ha, còn vi khuẩn và tảo sống cộng sinh có thể cố định được 150-200kg/ha/năm, trong khi đó quá trình tổng hợp hóa học trong tự nhiên chỉ là 3-5kg/ha. Trong tự nhiên, quá trình cố định N2 giữa vi khuẩn Rhizobium và rễ cây họ Đậu là quan trọng và có hiệu quả sinh học cao nhất.

1. Điều kiện cố định N2

Quá trình cố định nitơ cần phải có 4 điều kiện sau:
+ Có lực khử mạnh với thể năng khử cao (Ferredoxin, Plavodoxin, NAD hoặc NADP).
+ Có năng lượng đủ (ATP) và có sự tham gia của nguyên tố vi lượng (Mg).
+ Có sự tham gia của enzyme nitrogenase.
+ Phải tiến hành trong điều kiện yếm khí ( nồng độ O2 =0 hoặc gần bằng 0)

2. Mối quan hệ giữa Rhizobium và rễ cây họ Đậu

– Vi khuẩn Rhizobium có thể sống tự do trong đất, nhưng chúng lại không thể cố định nitơ ở trạng thái tự do và rễ cây họ đậu cúng không thể cố định N2 mà không có vi khuẩn.
– Sự cộng sinh giữa vi khuẩn Rhizobium và rễ cây họ Đậu dẫn đến các biến đổi cơ bản trong cấu trúc của rễ. Dọc theo mỗi rễ cây là các nốt phồng gọi là nốt sần bao gồm các tế bào thực vật đã bị nhiễm khuẩn.

III/. LƯƠNG DUYÊN RHIZOBIUM – CÂY HỌ ĐẬU

Rhizobium là một chi vi khuẩn Gram âm sống trong đất có vai trò cố định đạm. Rhizobium hình thành một nhóm vi khuẩn cộng sinh cố định đạm sống trong rễ của các cây họ Đậu (Rhizobium Legusminozarum).
Rhizobium xâm chiếm tế bào rễ của cây họ đậu, tạo thành các nốt sần ở rễ cây họ đậu, và định cư ở đó thực hiện quá trình cố định đạm. Cây thì cung cấp các hợp chất hữu cơ từ quá trình quang hợp như glucid, nguồn năng lượng và các chất khử như NADH2 để vi khuẩn hoạt động. Đây là hiệu ứng cộng sinh, làm cho cả hai (cây và vi khuẩn) cùng phát triển thuận lợi, ngoài ra sản phẩm dư thừa của quá trình cố định đạm còn cung cấp cho đất trồng, phục hồi và duy trì độ phì của đất, cải tạo và cải thiện môi trường trồng trọt.

Nhưng nói thế có lẽ cũng hơi oan uổng cho Rhizobium, vì thực ra thì “nàng” đậu đã “cua” chàng Rhizobium bằng cách tiết ra trên rễ một lọat các hợp chất như glucid, acid amin, các axit hữu cơ (axit malic, axit asparaginic,…) có tác dụng kích thích những chủng vi khuẩn nốt sần chuyên tính và ức chế những chủng không chuyên tính để thực hiện quá trình xâm nhiễm. Chàng Rhizobium “sập bẫy” nàng đậu, được cấp chỗ để xây dựng “loan phòng” là các nốt sần rồi mở nhà máy sản xuất phân đạm sinh học ở đó, tận tụy “nuôi” mỹ nhân họ Đậu.

 

IV/. NÔNG NGHIỆP RHIZOBIUM

Từ khi nhận thức được vai trò của “nhà máy sản xuất phân đạm sinh học rhizobium” thì con người đã có tư tưởng hướng đến một nền nông nghiệp nói không với phân hóa học. Tham vọng chung là “cấy” vi khuẩn nốt sần lên bộ rễ của các loài cây ngoài họ đậu như lúa mì, lúa nước, rau ăn lá, khoai tây hay cà chua, cà phê, ca cao v.v…

Tuy nhiên, có nhiều khó khăn khi tiếp cận vấn đề :
– Bộ rễ của các loài cây “không đậu” không sẵn sàng đón nhận Rhizobium với màng rễ dày, chắc.
– Cây chủ dùng hệ phản vệ của mình từ chối sự xâm nhập của Rhizobium, tiết ra các độc tố đẩy Rhizobium vào chỗ chết.
– Rhizobium không nhận được các chất hữu cơ và năng lượng cần thiết để duy trì sự sống và phục vụ cho quá trình cố định đạm.

Cho nên đến nay thì các loài cây ngoài họ đậu – Rhizobium vẫn chưa nghe tăm dạng. Khoa học Nông Nghiệp thế giới đã thất hứa và trễ hẹn nhiều lần, cho đến ngành giống cây trồng biến đổi GEN tuyên bố “xanh dờn” rồi cũng bó tay.

V/. RHIZOBIUM AGRICULTURE

Chúng tôi có 3 hướng thực hiện nông nghiệp không phân bón hóa học trên cơ sở Rhizobium như sau :

1/. Tập trung nghiên cứu, phân lập và sử dụng một họ Rhizobium đơn dòng, tên đầy đủ là PLASMO RHIZOBIUM LEGUSMINOZARUM có ưu tính hóa – sinh học đặc biệt đối với cây trồng, nhất là cây trồng có xuất xứ nhiệt đới, với emzime đặc hữu là nitrogenaza.. Từ đó tạo ra các chế phẩm Rhizobium phục vụ cho các mục tiêu chuyên biệt.

2/. Sử dụng các chế phẩm Rhizobium làm phân bón (rễ và lá).

2-a/. Trồng cây họ đậu có bộ rễ mang nốt sần lớn và chủng Rhizobium Legusminozarum mạnh có trong cây cây me đất (*) với cây rau rút dại. Sau khi thu hoạch rễ, sấy khô, nghiền rồi nhân sinh khối R.L. bằng cách trộn với phân hữu cơ sạch tiệt trùng. Sau thời gian nuôi ủ thì làm khô, nghiền mịn để có chế phẩm R.A.2a để bón. Rhizobium tiếp tục cố định đạm, cung cấp cho cây chủ.

2-b/. Dùng bột rễ, nuôi cấy trong môi trường nuôi cấy đặc trưng là nước thịt – Agar. Thực hiện thu họach chế phẩm R.A.2b này như ở 2-a.

Hai loại chế phẩm R.A.1a và R.A.1b nói trên có tác dụng tương tự như Nitrazin chế tạo ra lần đầu tiên ở Đức năm 1986.

3/. Dùng giá thể : Giá thể nốt sần họ đậu là giá đỗ xanh, trộn bột rễ, nuôi ủ 72 giờ trong nhiệt độ 40 độ C để chúng tạo kênh xâm nhập trên thân giá đỗ. Sau đó nghiền hỗn hợp để có chế phẩm R.A.2 bón cho cây chủ.

4/. Tạo nốt sần trên cây chủ :

– Xử lý rễ cây chủ bằng acid glucosic hay acid fructosic để làm “mềm” màng rễ cây chủ, tạo môi trường xâm nhập.
– Tẩm hỗn hợp sinh học bao gồm glucid + acid amin + các acid hữu cơ phù hợp để dẫn dụ Rhizobium.
– Bón chế phẩm R.A.2a; R.A.2b hay R.A.2 để cung cấp lượng lớn Rhizobium Legusminozarum xâm nhập và tạo nốt sần lên cây chủ.

Wambuakim

Xin xem tiếp phần II : Thảo luận về Nông Nghiệp Không Phân Bón..
===============================
(*) Me đất
Khả năng cố định đạm cũa R.L. me đất là cao nhất trong các chủng Rhizobium.(bản của Thomas Oester, Bremen 1998)
– Rhizo Đậu đũa, đậu răng ngựa: 45 – 552 kgN/ha/Kgnăm
– Rhizo Đậu Hà Lan 52 – 77 kgN/ha/Kgnăm
– Rhizo Đậu xanh 63 – 342 kgN/ha/Kgnăm
– Rhizo Đậu tương 179 kgN/ha/Kgnăm
– Rhizo Cây Medicago 504 kgN/ha/Kgnăm
– P.L. Me đất 1 tấn N/ha/Kgnăm

5.268 Trả lời “NÔNG NGHIỆP KHÔNG PHÂN BÓN PHẦN I”

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